LAUGHTER

This is a forwarded message that really made me laugh. I hope you will enjoy it.

Letter To Dad
A father passing by his teenage daughter's bedroom was astonished to see the bed was nicely made and everything was neat and tidy. Then he saw an envelope propped up prominently on the centre of the pillow. It was addressed "Dad". With the worst premonition, he opened the envelope and read the letter with trembling hands:-

Dear Dad,
It is with great regret and sorrow that I'm writing you, but I'm leaving home. I had to elope with my new boyfriend Randy because I wanted to avoid a scene with Mom and you. I've been finding real passion with Randy and he is so nice to me. I know when you meet him you'll like him too - even with all his piercing, tattoos, and motorcycle clothes. But it's not only the passion Dad, I'm pregnant and Randy said that he wants me to have the kid and that we can be very happy together. Even though Randy is much older than me (anyway, 42 isn't so old these days is it?), and has no money, really these things shouldn't stand in the way of our relationship, don't you agree?

Randy has a great CD collection; he already owns a trailer in the woods and has a stack of firewood for the whole winter. It's true he has other girlfriends as well but I know he'll be faithful to me in his own way. He wants to have many more children with me and that's now one of my dreams too.

Randy taught me that marijuana doesn't really hurt anyone and he'll be growing it for us and we'll trade it with our friends for all the cocaine and ecstasy we want. In the meantime, we'll pray that science will find a cure for AIDS so Randy can get better; he sure deserves it!!

Don't worry Dad, I'm 15 years old now and I know how to take care of myself. Someday I'm sure we'll be back to visit so you can get to know your grandchildren.

Your loving daughter,
Rosie.

At the bottom of the page were the letters "PTO". Hands still trembling, her father turned the sheet, and read:

PS: Dad, none of the above is true. I'm over at the neighbor’s house. I just wanted to remind you that there are worse things in life than my report card that's in my desk centre drawer. Please sign it and call when it is safe for me to come home. I love you

Distance, Time and Love

In loneliness, in sickness, in confusion in any situation the mere knowledge of you makes it possible to endure any problem even if you are powerless to help. It is enough that you exist in my life. Love is not diminished by imprisonment or war, by suffering or silence. Most importantly love is not faded out by distance and time. It is in these things that it roots most intensely. It is from these things that it flowers. It blooms and finally contents your life.

SILENT CURFEW

Happiness, pleasure, jubilance comes and goes and are the parts of life. Such moments are like the bursting of balloons. They make big noise but remains for a short time only. Life goes on and on. People forget these blissful moments. Among friends you will forget all those ecstatic moments which gave you contentment.

When you look back in life you will never remember your victory over your foe that gave you maximum pleasure. But you will always remember the silence of your friend. The same friend who was very dear to you, with whom you have shared your whole life. And whenever you think about your friend you will never remember those valued moments that made you laugh but you will always remember silence, betrayal and all those moments that made your heart cry.

Why do we think only about betrayal? Why only silence? Why cannot we be happy remembering gleeful moments; those moments which once were very precious to us. Why?

Perhaps this is the way of life. Maybe this is why we are humans and not God. You just have to let it go. And you just have to learn to live with it.

Cyclic AMP dependent Protein-Kinase (PKA)

cAMP exerts its effects mainly by activating cyclic AMP-dependent protein kinase (PKA).This enzyme catalyses the transfer of terminal P group from ATP to specific serine or threonines of selected target proteins.

In inactive state, PKA consists of two catalytic & two regulatory subunits ‘C2 R2’ with regulatory units blocking catalytic centers of catalytic units.The binding of cAMP to the regulatory subunit (2 on each regulatory subunits) alters their conformation, causing them to dissociate from the complex.The released catalytic subunits are thereby activated to phosphorylate specific substrate protein molecules.

TURN ON

Initially the G-protein a subunit has bound GDP, and the a, b, & y subunits are complexed together.

• Hormone binding to a 7-helix receptor (GPCR) causes a conformational change in the receptor that is transmitted to the G-protein. The nucleotide-binding site on G-a becomes more accessible to the cytosol. G-a releases GDP and binds GTP. (GDP-GTP exchange)


• Substitution of GTP for GDP causes another conformational change in G-a. Ga-GTP dissociates from the inhibitory b,y subunit complex, and can now bind to and activate Adenylyl Cyclase.


• Adenylyl Cyclase, activated by the stimulatory Ga-GTP, catalyzes synthesis of cAMP.


• Protein Kinase A (cAMP-Dependent Protein Kinase) catalyzes phosphorylation of various cellular proteins, altering their activity.
  

TURN OFF

• Ga hydrolyzes GTP to GDP + Pi (GTPase). The presence of GDP on Ga causes it to rebind to the inhibitory b,y complex. Adenylate cyclase is no longer activated.


• Phosphodiesterase catalyzes hydrolysis of cAMP to AMP.


• Receptor desensitization occurs. This process varies with the hormone. Some receptors are phosphorylated via specific receptor kinases. The phosphorylated receptor may then bind to a protein arrestin, that promotes removal of the receptor from the membrane by clathrin-mediated endocytosis.


• Protein Phosphatase catalyzes removal by hydrolysis of phosphates that were attached to proteins via Protein Kinase A.

THE RIGHT PERSON??

Many people believe (because we have long been told from movies) that there is a person who fits our specific requirements and it is just a case of finding him. Some people probably do. But most people in happy, stable relationships have partners who meet some of the important qualities rather than all of their requirements.

Supportive acceptance to each other’s shortcomings, real or perceived is the foundation of a relationship. After all nobody is perfect (Except Of Course “ME”). By rejecting any person you meet, who does not immediately appear absolutely right for you, you are missing out on knowing some wonderful people, one of whom could turn out to be “The One” for you.

Finding the “Right Person” is about finding yourself, knowing what you are and what is important to you. If fantastic looks are important to you that is fine but ultimately a person who makes you happy from day to day is more of an asset than the handsome hunk you would have preferred.

Many people are afraid of intimacy. This fear is closely related to the fear of abandonment and rejection. Perhaps these people think on a subconscious and unintentional level, that once he gets to know you he won’t like you. So you reject him before he can reject you. Take a hard, honest look at yourself and ask is this what you want. Do not think I’m not worthy. You should try to reinvent yourself and your place in the world. You should become whole before becoming somebody’s half.

Always feel confident about yourself. Love yourself. If you do not love yourself, the vibes you give off repel other person from you and it is a great inhibitor to being liked by others. Do not have any inferiority complex. Always believe in yourself. Remember it is you and your feeling that is important in your life.

ABOUT LOVE.....

It takes only a minute to get crush on someone, an hour to like someone and a day to love someone. We never find out from where love begins and where it ends. If love has its beginning and end then that’s not love, that’s only as attraction. If something is liked, but not loved it’s just an attraction. True love can only be felt, not seen.

It hurts to love someone and not to be loved in return, but what is more painful is to love someone but never find courage to let that person know how you feel.

Love is that when you take away the feelings, the passion and the romance in a relationship and find out you still care for that person. Sad things in life is when you meet someone who meant a lot to you but only to find out in the end that it was never meant to be yours and you just have to let it go.

Sometimes we find our heart broken, feelings shattered by those person whom we have once believed and trusted on. Maybe God wants us to meet a few wrong people before meeting the right one, so that when we finally meet the right person. We will know now to be grateful for that gift. When the door of happiness closes another door opens but we often look so long as the closed door that we don’t see the one, which has been opened for us.

Giving all your love to someone is never an assurance that he/she will also love you. Don’t expect love in return; just wait for it to grow in theirs heart. But if it doesn’t still be content it grew in yours.

A thought for today : FRIENDSHIP

Life means missing expected things and facing unexpected things. When you are right, no one remembers, but when you are wrong, no one forgets. This is life.

Keep the lamp of friendship burning with oil of love because sun rises in east and sets in west. But friendship rises in heart and sets after death. Do remember ur friends. They are really special in life.